Geisinger Medical Laboratories/Geisinger Proven Diagnostics Test Catalog

Order Display Name: Geisinger Epic Procedure Code Or ID CPT Code:  

PLASMINOGEN ACTIVITY

ORDERING INFORMATION:
Geisinger Epic Procedure Code: LAB2856         Geisinger Epic ID: 7179

SPECIMEN COLLECTION
Specimen type:
Plasma
Preferred collection container:
Specimen required:
2 mL aliquot of plasma; minimum 1 mL (frozen). Please submit a separate frozen aliquot for this test. It may not be combined with any other test requiring a frozen aliquot.
Special notes:
3.2% citrate plasma, shipped frozen, is the only acceptable sample type. All other anticoagulants (heparin, EDTA, oxalate) are NOT acceptable. Proper blood to anticoagulant ratio is required: Vacutainer(R) tubes must be filled to completion to ensure the proper 9:1 blood to anticoagulant ratio is achieved. Routine collection requires 4.5 mL blood added to 0.5 mL sodium citrate. For patients with normal hematocrits, no adjustment is necessary. If the patient has a known hematocrit above 55%, adjust the amount of anticoagulant in the collection tube before drawing the blood according to the NCCLS guidelines below.

NCCLS Guidelines: Anticoagulant vol. [x]=(100-hematocrit)/(595-hematocrit)x total vol. of anticoagulated blood required. Example: Patient hematocrit = 60% (100-60)/(595-60)x 5.0 = 0.37 mL sodium citrate for a 5 mL standard drawing tube.

To avoid contaminating the sample with tissue thromboplastin or heparin, follow the guidelines below. These substances may alter results: The venipuncture must be clean, with no trauma. Hemolyzed samples are not acceptable. The first 5 mL of blood drawn from a patient should not be used for coagulation testing. If drawn through an indwelling catheter, flush with 5 mL of saline and discard the first 5 mL of blood collected before collecting the specimen for coagulation testing. Blood should not be collected from heparinized lines.

SPECIMEN PROCESSING
Specimen processing instructions:
Mix the sample gently by inverting the tube gently at least but not more than four times immediately after filling. Process the sample as soon as possible (within 60 minutes). Spin down the specimen at a speed and time required to produce platelet-poor plasma (< 5,000 - 10,000/uL). This can be accomplished by centrifuging at 1500 x g for 15 minutes. Transfer plasma into a plastic tube using a plastic Pasteur pipette. Do not use glass tubes or glass Pasteur pipettes as glass can activate the clotting cascade. Label each tube plasma. Submit a plasma aliquot for each and every coagulation assay requested (one tube for each test). If possible, submit one additional plasma aliquot for repeat and/or test additions. If you are requesting other tests that require serum, please label these tubes as serum. 
Transport temperature:
Frozen.
Specimen stability:
Room temperature: Unacceptable. Refrigerated: 2 hours. Frozen: 1 month (-70°C).
Rejection criteria:
Specimens transported at room temperature. Stability limits exceeded. Hemolyzed samples are not acceptable. Heparin, EDTA or oxalate anticoagulant.

TEST DETAILS
Reference interval:
65-176%
Additional information:
Set up Monday Wednesday, Friday; report available next day.
CPT code(s):
85420
Note: The billing party has sole responsibility for CPT coding.  Any questions regarding coding should be directed to the payer being billed.  The CPT codes provided by GML are based on AMA guidelines and are for informational purposes only.
Test includes:
Plasminogen activity.
Methodology:
Chromogenic substrate
Synonyms:
Quest test code 19333, PLACT
Clinical significance:
The precursor of plasmin is plasminogen, plasmin lyses fibrin clots. Activity is increased in pregnancy and as an acute phase reactant. Rare hereditary deficiency of plasminogen predisposes to venous thrombosis. Low activity is associated with DIC, liver disease, and increased risk of thrombosis.

Performing Locations

Quest Diagnostics

Technical Lead: Michael Weaver    
Frequency: Monday-Friday evening    
Performed stat? No
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