Geisinger Medical Laboratories/Geisinger Proven Diagnostics Test Catalog

Order Display Name: Geisinger Epic Procedure Code Or ID CPT Code:  

PLASMINOGEN ACTIVITY

ORDERING INFORMATION:
Geisinger Epic Procedure Code: LAB2856         Geisinger Epic ID: 7179

SPECIMEN COLLECTION
Specimen type:
Plasma
Preferred collection container:
Specimen required:
2 mL aliquot of frozen platelet-poor plasma; minimum 0.5 mL. Please submit a separate frozen aliquot for this test. It may not be combined with any other test requiring a frozen aliquot.
Special notes:
3.2% citrate plasma, shipped frozen, is the only acceptable sample type. All other anticoagulants (heparin, EDTA, oxalate) are NOT acceptable. Proper blood to anticoagulant ratio is required: Vacutainer(R) tubes must be filled to completion to ensure the proper 9:1 blood to anticoagulant ratio is achieved. Routine collection requires 4.5 mL blood added to 0.5 mL sodium citrate. For patients with normal hematocrits, no adjustment is necessary. If the patient has a known hematocrit above 55%, adjust the amount of anticoagulant in the collection tube before drawing the blood according to the NCCLS guidelines below.

NCCLS Guidelines: Anticoagulant vol. [x]=(100-hematocrit)/(595-hematocrit)x total vol. of anticoagulated blood required. Example: Patient hematocrit = 60% (100-60)/(595-60)x 5.0 = 0.37 mL sodium citrate for a 5 mL standard drawing tube.

To avoid contaminating the sample with tissue thromboplastin or heparin, follow the guidelines below. These substances may alter results: The venipuncture must be clean, with no trauma. Hemolyzed samples are not acceptable. The first 5 mL of blood drawn from a patient should not be used for coagulation testing. If drawn through an indwelling catheter, flush with 5 mL of saline and discard the first 5 mL of blood collected before collecting the specimen for coagulation testing. Blood should not be collected from heparinized lines.

SPECIMEN PROCESSING
Specimen processing instructions:
Please submit a separate, frozen vial for each special coagulation assay ordered. Draw blood in a light blue-top tube containing 3.2% sodium citrate, mix gently by inverting 3-4 times. Centrifuge 15 minutes at 1500 g within one hour of collection. Using a plastic pipette, remove plasma, taking care to avoid WBC/platelet buffy layer and place into a plastic vial. Centrifuge a second time and transfer platelet-poor plasma into a new plastic vial(s). Freeze immediately and ship on dry ice. 
Transport temperature:
Frozen.
Specimen stability:
Room temperature: Unacceptable. Refrigerated: Unacceptable. Frozen: 1 month (-70°C).
Rejection criteria:
Specimens transported at room temperature or refrgierated. Stability limits exceeded. Gross hemolysis • Grossly lipemic • Grossly icteric.

TEST DETAILS
CPT code(s):
85420
Note: The billing party has sole responsibility for CPT coding.  Any questions regarding coding should be directed to the payer being billed.  The CPT codes provided by GML are based on AMA guidelines and are for informational purposes only.
Test includes:
Plasminogen activity.
Methodology:
Chromogenic substrate
Synonyms:
Quest test code 4458, PLACT
Clinical significance:
The precursor of plasmin is plasminogen, plasmin lyses fibrin clots. Activity is increased in pregnancy and as an acute phase reactant. Rare hereditary deficiency of plasminogen predisposes to venous thrombosis. Low activity is associated with DIC, liver disease, and increased risk of thrombosis.

Performing Locations

Quest Diagnostics

Technical Lead: Michael Weaver    
Frequency: Setup: Monday, Wednesday, Friday. Report available: 1-3 days.    
Performed STAT? No
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