Geisinger Medical Laboratories/Geisinger Proven Diagnostics Test Catalog

Test Name: Sunquest Test Code Epic Procedure Code Or ID CPT Code:  

HCV GENOTYPE, TRANSPLANT DECEASED DONOR TESTING

ORDERING INFORMATION:
Sunquest Test Code:
DHCGEN      Geisinger Epic Procedure Code: DHCGEN         Geisinger Epic ID: 47644

SPECIMEN COLLECTION
Specimen type:
Plasma
Preferred collection container:
Specimen required:
3-4 mL of plasma in one (1) aliquot tube; minimum 2.4 mL of plasma in aliquot. NO SHARED SPECIMENS.
Special notes:
This test is only used for DECEASED DONOR TESTING. The donor sample is ordered under the recipient's name and medical record number (MRN).

SPECIMEN PROCESSING
Specimen processing instructions:
Separate plasma from whole blood within 24 hours of collection. Each molecular test ordered requires its own aliquot tube (e.g. DHEPCR requires one 3-4 mL aliquot tube and DHCGEN requires on 3-4 mL aliquot tube). 
Transport temperature:
Plasma frozen, -20 to -80ºC (preferred). Plasma may also be transported at 2-8ºC. Transport EDTA whole blood at 2-8ºC.
Specimen stability:
Plasma specimens may be stored at 2-8ºC for up to 72 hours, or frozen at -20 to -80ºC up to 6 weeks (preferred). EDTA whole blood specimens can be stored at 2-8ºC up to 24 hours prior to centrifugation.
Rejection criteria:
Whole blood specimens must be collected in EDTA tubes. Specimens must be separated within 24 hours, or they will be rejected. Plasma must be stored at 2-8ºC for up to 72 hours or at -20 to -80ºC up to 6 weeks. Stability limits exceeded. Shared or comingled specimens.

TEST DETAILS
Reference interval:
Negative.
Additional information:
The LOD for HCV Genotypes 1-5 is less than or equal to 500 IU/mL. RNA quantitations lower than 500 IU/mL can produce a valid genotype, unable to genotype, or HCV not detected. The LOD of HCV Genotype 6 is less than or equal to 1000 IU/mL. RNA quantitations lower than 1000 IU/mL can produce a valid genotype, unable to genotype, or HCV not detected.
CPT code(s):
87902
Note: The billing party has sole responsibility for CPT coding.  Any questions regarding coding should be directed to the payer being billed.  The CPT codes provided by GML are based on AMA guidelines and are for informational purposes only.
Test includes:
Hepatitis C Genotyping.
Methodology:
Multiplex Reverse-Transcription Polymerase Chain Reaction (RT-PCR)
GenMark Dx eSensor Technology
Synonyms:
HCV genotyping, HCV RNA genotyping, HCV, Hepatitis C virus genotyping, Hepatitis C genotyping
Clinical significance:
This test detects the six major HCV genotypes and their most common subtypes. The transplant deceased donor hepatitis C genotype test provides the HCV genotype in donor blood samples.
Doctoral Director(s:)
Yi Ding MD, PhD    
Review Date: 03/06/2019

Performing Locations

Geisinger Medical Center

Performing laboratory: Molecular Diagnostics
Technical Lead: Lisa Scicchitano
Frequency: Twice weekly
Performed stat? No
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