Geisinger Medical Laboratories Test Catalog

Order Display Name: Geisinger Epic Procedure Code Or ID CPT Code:  

SCID RELATED LYMPHOCYTE SUBSETS

ORDERING INFORMATION:
Geisinger Epic Procedure Code: LAB3033         Geisinger Epic ID: 47611

SPECIMEN COLLECTION
Specimen type:
 Whole blood
Preferred collection container:
Specimen required:
Two (2) properly drawn K2 EDTA tubes whole blood.
Special notes:
 Collect TWO tubes. Do not combine tubes. Only order if patient is under 2 years of age. If patient is over 2 years of age, order T4T8 lymphocyte subset panel, flow cytometry
SPECIMEN PROCESSING
Specimen processing instructions:
Do not centrifuge, refrigerate or freeze. 
Transport temperature:
 Room temperature. Do not expose to extremes of temperature.
Specimen stability:
 48 hours at room temperature.
Rejection criteria:
 Specimen stability exceeded. Clotted specimen. Hemolyzed specimen.
TEST DETAILS
Reference interval:
AGE: CD3 % CD3+
CD4%		 CD3+
CD8%		 CD19 % CD56+
CD16 %		 CD4:CD8 RATIO
17- adult 61-88 35-62 13-35 4.0-21 3.0-21 0.8-3.5
10 52-78 25-48 9.0-35.0 8.0-24.0 6.0-27. 0.9-3.4
5 55-78 27-53 19.0-34.0 10.0-31.0 4.0-26.0 0.9-2.6
2 43-76 23-48 14.0-33.0 14.0-44.0 4.0-23.0 0.9-2.9
15 M 39-73 25-50 11.0-32.0 17.0-41.0 3.0-16.0 0.9-3.7
9 M 54-76 31-54 12.0-28.0 15.0-39.0 3.0-17.0 1.3-3.9
5 M 50-77 33-58 13.0-26.0 13.0-35.0 2.0-13.0 1.6-3.8
2 M 48-75 33-58 11.0-25.0 14.0-39.0 2.0-14.0 1.7-3.9
7 D 60-85 41-68 9.0-23.0 4.0-26.0 3.0-23.0 1.3-6.3
0 28-76 17-52 10.0-41.0 5.0-22.0 6.0-58.0 1.0-2.6

AGE: CD3 Absolute CD3+/CD4+ Absolute CD3+/CD8+ Absolute CD19/CD3-
Absolute		 CD56+CD16 Absolute
17 - adult 510-2265 330-1520 150-790 33-550 50-400
10 800-3500 400-2100 200-1200 200-600 70-1200
5 700-4200 300-2000 300-1800 200-1600 90-900
2 900-4500 500-2400 300-1600 200-2100 100-1000
15 M 1400-8000 900-5500 400-2300 600-3100 100-1400
9 M 1600-6700 1000-4600 400-2100 600-2700 200-1200
5 M 2400-6900 1400-5100 600-2200 700-2500 100-1000
2 M 2300-6500 1500-5000 500-1600 600-3000 100-1300
7 D 2300-7000 1700-5300 400-1700 60-1900 200-1400
0 600-5000 400-3500 200-1900 40-1100 100-1900

*Adult ranges established by normal study performed at GMC 2002
*Pediatric ranges from : The Journal of Pediatrics Vol. 130(3), March 1997, pp 388-393
Interpretation guide:
 In infant under one year of age further clinical evaluation is recommended if the absolute CD3 is less than 1500 lymphs/uL.
Additional information:
 Analysis performed according to CDC Guidelines.
CPT code(s):
 86355 86357 86359 86360
Note: The billing party has sole responsibility for CPT coding.  Any questions regarding coding should be directed to the payer being billed.  The CPT codes provided by GML are based on AMA guidelines and are for informational purposes only.
Test includes:
 Total T Cells, T-helper cells, T- suppressor cells, B cells and NK (natural killer) cells.
Methodology:
Flow Cytometry
Synonyms:
Severe combined immunodefiency by flow cytometry (SCID by flow)
SCIDF
Clinical significance:
Lymphocyte subset anlysis is useful for evaluation of immunocompetency in suspected cases of congenital or acquired immunodeficiency.
Doctoral Director(s:)
Kai Zhang M.D.    
Review Date: 12/14/2022

Performing Locations

Geisinger Medical Center

Performing laboratory: Flow Cytometry
Technical Lead: Michele Adler
Frequency: 1st shift 7am to 5pm. Call 570-271-5261 during hours of operation,
Performed STAT? No
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