Geisinger Medical Laboratories/Geisinger Proven Diagnostics Test Catalog

Test Name: Sunquest Test Code Epic Procedure Code Or ID CPT Code:  

BRAF MUTATION TESTING

ORDERING INFORMATION:
Sunquest Test Code:
     Geisinger Epic Procedure Code: BRAFMU         Geisinger Epic ID: 41315

SPECIMEN COLLECTION
Specimen type:
Formalin-fixed, paraffin-embedded tissue (FFPE)
Specimen required:
Two (2) 15 micron sections in 50 mL tube (preferred), four (4) 10 micron FFPE unstained slides with H&E or paraffin block.
Special notes:
For requests from non-Geisinger sites, please send: Five (5) 15 micron FFPE sections (scrolls)-preferred specimen, H&E slide, source of specimen, and tumor assessment (<10% or >10%). Alternatively, send FFPE block. Isolated/extracted nucleic acids are acceptable only from CLIA-certified laboratories or a laboratory meeting equivalent requirements as determined by CAP and/or CMS.

SPECIMEN PROCESSING
Transport temperature:
Room temperature (18-25°C).
Specimen stability:
Indefinitely.
Rejection criteria:
Blocks containing no tumor (evaluted by pathologist) will be rejected. FFPE tissues that have been decalcified. Insufficient DNA.

TEST DETAILS
Reference interval:
Negative for mutation tested.
Additional information:
Abundance of melanin pigment may interfere with the assay.
CPT code(s):
81210
Note: The billing party has sole responsibility for CPT coding.  Any questions regarding coding should be directed to the payer being billed.  The CPT codes provided by GML are based on AMA guidelines and are for informational purposes only.
Test includes:
Identification of BRAF mutations in codon 600. (1) Colorectal or metastasized colorectal cancer - V600E/D or V600K/R; (2) All other cancer types - V600E/E2/D or V600K/R/M.
Methodology:
Real-time Polymerase Chain Reaction (PCR)
Synonyms:
BRAF; V-RAF murine sarcome viral oncogene homolog B1, proto-oncogen B-Raf, serine/threonine-protein kinase B-Raf
Clinical significance:
Detect activating BRAF mutations at codon 600, which can indicate responsiveness to BRAF inhibitors in melanomas or resistance to anti-EGFR therapy in colorectal cancer. Useful in assessing prognosis of certain thyroid cancers. Can also be used within the Lynch syndrome reflex testing pathway (for colorectal cancer specimens only).
Doctoral Director(s:)
Yi Ding MD, PhD    
Review Date: 03/29/2019

Performing Locations

Geisinger Medical Center

Performing laboratory: Molecular Diagnostics
Technical Lead: Lisa Scicchitano
Frequency: Daily, Monday-Friday
Performed stat? No
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