Geisinger Medical Laboratories/Geisinger Proven Diagnostics Test Catalog

Order Display Name: Geisinger Epic Procedure Code Or ID CPT Code:  

FACTOR VIII (8) CLOTTING ACTIVITY

ORDERING INFORMATION:
Geisinger Epic Procedure Code: LAB2257         Geisinger Epic ID: 7123

SPECIMEN COLLECTION
Specimen type:
Platelet-free plasma
Preferred collection container:
Alternate Collection Container:
Other size blue-top (3.2% sodium citrate) tubes (e.g., 1.8 mL, 4.5 mL)
Specimen required:
1 mL aliquot plasma.
Special notes:
Do not draw blood from a heparinized line. Avoid contaminating the sample with tissue thromboplastin or heparin. Venipuncture must be performed with no trauma. If blood is drawn from an indwelling catheter, flush with 5 mL of saline and discard the first 5 mL of blood collected. If drawn with a butterfly setup, draw a discard first to remove air from tubing.

SPECIMEN PROCESSING
Specimen processing instructions:
Centrifuge the citrated blue-top tubes as soon as possible (within 60 minutes) at designated time and speed to obtain platelet poor plasma (<10,000/µL). Using a plastic pipette, remove 1 mL of plasma and transfer to a plastic polypropylene aliquot tube and label with patient's name and a second patient identifier (e.g., date of birth, medical record number). Freeze upright and transport on dry ice. 
Transport temperature:
Frozen.
Specimen stability:
Frozen: -20°C for 30 days, -70°C for 6 months. Room temperature: 4 hours.
Rejection criteria:
Clotted specimen. Stability limits exceeded. Overfilled or underfilled tubes. HCT > 55 requires rejection, reorder, and recollection with proper adjustment of anticoagulant. Indications of thaw in transit are sample on slant in aliquot tube or in lid of tube. These samples will be run but will receive a comment of interpret results with caution as thawing suspected.

TEST DETAILS
Reference interval:
GMC: 55-145%
GWV: 60-150%
Additional information:
Anticoagulation Impact on Coagulation Tests
CPT code(s):
85240
Note: The billing party has sole responsibility for CPT coding.  Any questions regarding coding should be directed to the payer being billed.  The CPT codes provided by GML are based on AMA guidelines and are for informational purposes only.
Test includes:
Factor VIII activity.
Methodology:
Mechanical Clot-Based
Synonyms:
Factor VIII procoagulant, Factor VIII Activity, Factor 8 Assay, Antihemophilic, FA8, Factor VIII Assay
Clinical significance:
Factor VIII assay is useful in evaluating the reason for a prolonged APTT test. Low levels are seen in hereditary and acquired hemophilia A and von Willebrand disease. Elevated levels may be associated with thrombophilia or reactive processes. Blood type has an influence on this value.
Doctoral Director(s:)
Michelle Grant DO    
Review Date: 10/24/2019

Performing Locations

Geisinger Medical Center

Performing laboratory: Coagulation
Technical Lead: Margaret Knowles-Tuchman
Frequency: Daily, all shifts
Performed stat? Yes

Geisinger Wyoming Valley Medical Center

Performing laboratory: ATL+
Technical Lead: Julia Bell    
Frequency: Daily, all shifts    
Performed stat? Yes
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