Geisinger Medical Laboratories/Geisinger Proven Diagnostics Test Catalog

Order Display Name: Geisinger Epic Procedure Code Or ID CPT Code:  

APTT, HEPARIN NEUTRALIZED

ORDERING INFORMATION:
Geisinger Epic Procedure Code: LAB1773         Geisinger Epic ID: 31808

SPECIMEN COLLECTION
Specimen type:
Platelet-free plasma
Preferred collection container:
Alternate Collection Container:
Other size blue-top (3.2% sodium citrate) tubes (e.g., 1.8 mL, 4.5 mL)
Specimen required:
1 mL aliquot of plasma
Special notes:
Do not draw blood from a heparinized line. Avoid contaminating the sample with tissue thromboplastin or heparin. Venipuncture must be performed with no trauma. If blood is drawn from an indwelling catheter, flush with 5 mL of saline and discard the first 5 mL of blood collected. If blood is drawn with a butterfly device, draw a discard tube first to remove air from tubing.

SPECIMEN PROCESSING
Specimen processing instructions:
Centrifuge the citrated blue-top tubes as soon as possible (within 60 minutes) at designated time and speed to obtain platelet-poor plasma (<10,000/µL). Using a plastic pipette, remove 1 mL of plasma and transfer to a plastic polypropylene aliquot tube and label with patient's name and a second patient identifier (e.g., date of birth, medical record number). Freeze upright and transport on dry ice. 
Transport temperature:
Frozen.
Specimen stability:
Frozen: -20°C for 30 days, -70°C for 6 months. Room temperature: 4 hours. Stable 2 hours at room temperature if patient is on heparin.
Rejection criteria:
Clotted plasma. Underfilled or overfilled tubes. HCT >55. Stability limits exceeded. Indications of thaw in transit are sample on slant in aliquot tube or in lid of tube. These samples will be run but will receive a comment of interpret results with caution as thawing suspected.

TEST DETAILS
Reference interval:
21-38 seconds.
Critical values/courtesy alerts:
Greater than 120 seconds.
Additional information:
Anticoagulation Impact on Coagulation Tests
CPT code(s):
85730
Note: The billing party has sole responsibility for CPT coding.  Any questions regarding coding should be directed to the payer being billed.  The CPT codes provided by GML are based on AMA guidelines and are for informational purposes only.
Test includes:
APTT.
Methodology:
Mechanical Clot-Based
Synonyms:
PTH, Partial Thromboplastin Time, Activated Partial Thromboplastion Time (APTT) with Heparin Absorption, APTTH
Clinical significance:
Heparin can falsely elevated aPTT. This test removes heparin from specimen before APTT testing. High levels of heparin may not be completely removed.
Doctoral Director(s:)
Michelle Grant DO    
Review Date: 01/11/2020

Performing Locations

Geisinger Medical Center

Performing laboratory: Coagulation
Technical Lead: Margaret Knowles-Tuchman
Frequency: Daily, all shifts
Performed stat? Yes

Geisinger Wyoming Valley Medical Center

Performing laboratory: ATL+
Technical Lead: Julia Bell    
Frequency: Daily, all shifts    
Performed stat? Yes
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