Geisinger Medical Laboratories/Geisinger Proven Diagnostics Test Catalog

Test Name: Sunquest Test Code Epic Procedure Code Or ID CPT Code:  

APTT, HEPARIN NEUTRALIZED

ORDERING INFORMATION:
Sunquest Test Code:
APTTH      Geisinger Epic Procedure Code: APTTH         Geisinger Epic ID: 31808

SPECIMEN COLLECTION
Specimen type:
Platelet-free plasma
Preferred collection container:
Alternate Collection Container:
Other size blue-top (3.2% sodium citrate) tubes (e.g., 1.8 mL, 4.5 mL)
Specimen required:
1 mL aliquot of plasma
Special notes:
Do not draw blood from a heparinized line. Avoid contaminating the sample with tissue thromboplastin or heparin. Venipuncture must be performed with no trauma. If blood is drawn from an indwelling catheter, flush with 5 mL of saline and discard the first 5 mL of blood collected. If blood is drawn with a butterfly device, draw a discard tube first to remove air from tubing.NOTE: Test sample cannot be hepasorbed (enzyme that degrades heparin) if patient is on therapeutic heparin therapy. Questions are to be directed to the coagulation laboratory.
Patient preparation:
None.

SPECIMEN PROCESSING
Specimen processing instructions:
Centrifuge the citrated blue-top tubes as soon as possible (within 60 minutes) at designated time and speed to obtain platelet-poor plasma (<10,000/µL). Using a plastic pipette, remove 1 mL of plasma and transfer to a plastic aliquot tube and label with patient's name and a second patient identifier (e.g., date of birth, medical record number). 
Transport temperature:
Frozen.
Specimen stability:
Frozen: -20°C for 30 days, -70°C for 6 months. Room temperature: 4 hours. Stable 2 hours at room temperature if patient is on heparin.
Rejection criteria:
Clotted plasma. Underfilled or overfilled tubes. HCT >55. Stability limits exceeded.

TEST DETAILS
Reference interval:
21-38 seconds. Varies with lot number of reagent.
Critical values/courtesy alerts:
Greater than 120 seconds.
Additional information:
Plasma will be pretreated with heparin removal reagent before testing.
CPT code(s):
85730
Note: The billing party has sole responsibility for CPT coding.  Any questions regarding coding should be directed to the payer being billed.  The CPT codes provided by GML are based on AMA guidelines and are for informational purposes only.
Test includes:
APTT.
Methodology:
Mechanical Clot-Based
Synonyms:
PTH, Partial Thromboplastin Time, Activated Partial Thromboplastion Time (APTT) with Heparin Absorption
Clinical significance:
Preanalytical treatment of a plasma sample to remove trace heparin from a specimen for APTT testing.
Doctoral Director(s:)
Michelle Grant DO    
Review Date: 04/29/2019

Performing Locations

Geisinger Medical Center

Performing laboratory: Coagulation
Technical Lead: Margaret Knowles-Tuchman
Frequency: Daily, all shifts
Performed stat? Yes

Geisinger Wyoming Valley Medical Center

Performing laboratory: ATL+
Technical Lead: Amanda Mika    
Frequency: Daily, all shifts    
Performed stat? Yes
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*GMC - Geisinger Medical Center; GWV - Geisinger Wyoming Valley Medical Center; GSACH - Geisinger Shamokin Area Community Hospital; GBH - Geisinger Bloomsburg Hospital;
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