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Geisinger Medical Laboratories/Geisinger Proven Diagnostics Test Catalog
APTT |
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ORDERING INFORMATION: |
Geisinger Epic Procedure Code: LAB1772 Geisinger Epic ID: 7231 | |
SPECIMEN COLLECTION |
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Specimen type: |
Platelet-free plasma. | |
Preferred collection container: |
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Alternate Collection Container: |
Other size blue-top (3.2% sodium citrate) tubes (e.g., 1.8 mL, 4.5 mL)
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Specimen required: |
1 mL aliquot plasma. | |
Special notes: |
Do not draw blood from a heparinized line. Avoid contaminating the sample with tissue thromboplastin or heparin. Venipuncture must be performed with no trauma. If blood is drawn from an indwelling catheter, flush with 5 mL of saline and discard the first 5 mL of blood collected. NOTE: Test sample cannot be hepasorbed (enzyme that degrades heparin) if patient is on therapeutic heparin therapy. Questions are to be directed to the coagulation laboratory at GMC. If blood is drawn with a butterfly device, draw a discard tube first to remove air from tubing. | |
| SPECIMEN PROCESSING |
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Specimen processing instructions: |
Centrifuge the citrated blue-top tubes as soon as possible (within 60 minutes) at designated time and speed to obtain platelet-poor plasma (<10,000/µL). Using a plastic pipette, remove 1 mL of plasma and transfer to a plastic polypropylene aliquot tube and label with patient's name and a second patient identifier (e.g., date of birth, medical record number). Freeze upright and transport on dry ice. | |
Transport temperature: |
Frozen. | |
Specimen stability: |
Frozen: -20°C for 30 days, -70°C for 6 months. Room temperature: 4 hours. Stable 2 hours at room temperature if patient is on heparin. | |
Rejection criteria: |
Clotted specimen. Underfilled or overfilled tubes. Stability limits exceeded. HCT >55. HCT > 55 requires rejection, reorder in LIS, and recollection with proper adjustment of anticoagulant. Indications of thaw in transit are sample on slant in aliquot tube or in lid of tube. These samples will be run but will receive a comment of interpret results with caution as thawing suspected. | |
TEST DETAILS |
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Reference interval: |
21-38 seconds. | |
Critical values/courtesy alerts: |
Greater than 120 seconds. | |
Additional information: |
Anticoagulation Impact on Coagulation Tests | |
CPT code(s): |
85730 | |
| Note: The billing party has sole responsibility for CPT coding. Any questions regarding coding should be directed to the payer being billed. The CPT codes provided by GML are based on AMA guidelines and are for informational purposes only. | ||
Test includes: |
APTT. | |
Methodology: |
Mechanical Clot-Based |
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Synonyms: |
PTT, PARTIAL THROMBOPLASTIN TIME, APTT, PARTIAL, PLASMA ACTIVATED PARTIAL THROMBOPLASTIN TIME
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Clinical significance: |
APTT used to assess the intrinsic clotting pathway. Prolongation of the APTT may be associated with bleeding and may be due to a factor deficiency(ies) or factor inhibitor(s). The APTT test should not be used to monitor the effect of therapeutic unfractionated heparin instead an unfractionated heparin level should be ordered. | |
Doctoral Director(s:) |
Michelle Grant DO | |
| Review Date: | 01/11/2020 | |
Performing Locations
Geisinger Wyoming Valley Medical Center
Geisinger Shamokin Area Community Hospital
Geisinger Community Medical Center