Geisinger Medical Laboratories/Geisinger Proven Diagnostics Test Catalog

Order Display Name: Geisinger Epic Procedure Code Or ID CPT Code:  

PARVOVIRUS B19 DNA, QL REAL TIME PCR

ORDERING INFORMATION:
Geisinger Epic Procedure Code: LAB2816         Geisinger Epic ID: 17685

SPECIMEN COLLECTION
Specimen type:
Plasma (preferred), serum, tissue, amniotic fluid, or bone marrow.
Preferred collection container:
Alternate Collection Container:
Sterile specimen container
Sterile screw-cap tube
Specimen required:
1 mL plasma (preferred) or serum or whole blood, or bone marrow or amniotic fluid or 3 mm tissue.
Special notes:
Plasma (preferred): Collect blood in sterile tubes containing EDTA as anticoagulant or in Plasma Preparation Tubes (PPTs). Heparinized specimens are unacceptable.
Collect whole blood or bone marrow in sterile tubes containing EDTA as anticoagulant.
Amniotic fluid: Collect in a sterile, leakproof container.
Tissue: Collect in a sterile, leakproof container.
Serum: Collect blood in sterile tubes without anticoagulants; serum separator tube is recommended.
Tissue: Collect in a sterile, leakproof container.

SPECIMEN PROCESSING
Specimen processing instructions:
Plasma: Store collected whole blood at room temperature and separate plasma from cells within 2 hours of collection. Transfer plasma to sterile, plastic, screw-capped tubes and store refrigerated or frozen. If blood is collected in a PPT tube, centrifuge within 2 hours of collection. Transfer plasma to sterile, plastic, screw-capped tubes and store refrigerated or frozen. If blood is collected in a PPT tube, centrifuge within 2 hours of collection and store refrigerated or frozen. It is not necessary to transfer the plasma from a PPT tube to aliquot tubes.

Serum: Allow blood to clot at room temperature and separate serum from cells within 2 hours of collection. Transfer serum to sterile, plastic, screw-capped aliquot tubes. Store refrigerated up to 7 days or frozen -18 degrees C or lower for up to 30 days. Avoid repeated freezing and thawing of specimen. 

Transport temperature:
Refrigerated: Whole Blood, Bone Marrow, Amniotic Fluid, Serum, Urine. Frozen: Plasma, Tissue.
Specimen stability:
Plasma: Room Temperature: 48 hours. Refrigerated: 7 days. Frozen: 30 days.
Serum: Room Temperature: 48 hours. Refrigerated: 7 days. Frozen: 30 days.
Whole blood or bone marrow: Room Temperature: 48 hours. Refrigerated: 7 days.Frozen: Unacceptable.
Amniotic Fluid: Room Temperature: 48 hours. Refrigerated: 7 days. Frozen: 30 days.
Tissue: Room Temperature: 48 hours. Refrigerated: 7 days. Frozen: 30 days.
Rejection criteria:
Heparinized specimens are not acceptable. Stability limits exceeded. CSF and synovial fluid are not acceptable. Hemolyzed whole blood unacceptable.

TEST DETAILS
Reference interval:
Not detected.
CPT code(s):
87798
Note: The billing party has sole responsibility for CPT coding.  Any questions regarding coding should be directed to the payer being billed.  The CPT codes provided by GML are based on AMA guidelines and are for informational purposes only.
Test includes:
Parvovirus B19 DNA, Qualitative Real-Time PCR.
Methodology:
Real-time Polymerase Chain Reaction (PCR)
Synonyms:
PRVD, Parvovirus B19 DNA PCR
Quest test code 5319
Clinical significance:
Parvovirus B19 infection causes "Fifth Disease," a rash illness of childhood characterized by a bright red "slapped check" rash and a lacy rash on the trunk and limbs. Joint pain and swelling is more common in adults. Individuals with chronic hemolytic anemia such as sickle cell anemia may develop transient aplastic crisis with severe anemia. Immunocompromised individuals may develop chronic or recurrent bone marrow suppression. Infection during pregnancy can lead to fetal hydrops and spontaneous abortion. DNA testing in conjunction with B19 specific serology provides the most reliable evidence of a recent infection.

Performing Locations

Quest Diagnostics

Technical Lead: Michael Weaver    
Frequency: Monday-Sunday morning    
Performed stat? No
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