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ACTIVATED PROTEIN C RESISTANCE (FACTOR V LEIDEN CLOT BASED SCREENING ASSAY) |
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ORDERING INFORMATION: |
Geisinger Epic Procedure Code: LAB1515 Geisinger Epic ID: 19435 | |
SPECIMEN COLLECTION |
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Specimen type: |
Platelet-free plasma | |
Preferred collection container: |
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Alternate Collection Container: |
Other 3 mL blue-top (3.2% sodium citrate) tube
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Specimen required: |
2 mL aliquot of frozen, platelet-poor plasma, (minimum 1 ml) | |
Special notes: |
Please submit a separate frozen aliquot for this test. It may not be combined with any other test requiring a frozen aliquot. | |
| SPECIMEN PROCESSING |
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Specimen processing instructions: |
Centrifuge light blue-top tube 15 minutes at approximately 1500 x g within 60 minutes of collection. Using a plastic pipette, remove plasma, taking care to avoid the WBC/platelet buffy layer and place into a plastic vial. Centrifuge a second time and transfer platelet-poor plasma into a new plastic vial. Plasma must be free of platelets (< 10,000/mcL). Freeze immediately and ship on dry ice. | |
Transport temperature: |
Frozen. | |
Specimen stability: |
Room temperature: Unacceptable. Refrigerated: Unacceptable. Frozen:30 days | |
Rejection criteria: |
Received room temperature • Received refrigerated • Clotted specimen • Serum • Gross hemolysis | |
TEST DETAILS |
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CPT code(s): |
85307 | |
| Note: The billing party has sole responsibility for CPT coding. Any questions regarding coding should be directed to the payer being billed. The CPT codes provided by GML are based on AMA guidelines and are for informational purposes only. | ||
Methodology: |
RVVT-Based Assay (clot-based) |
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Synonyms: |
Quest test code 22, APC, APC Resistance, Resistance to Activated Protein C, ACTIPROTCRES
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Clinical significance: |
The activated protein C resistance test is a clot-based screening test used to - Activated protein C (APC) resistance is the most frequent hereditary defect associated with deep vein thrombosis. Over 95% of the APC resistance phenotype is due to the Factor V Leiden mutation. The APC-Resistance assay is a plasma based functional test for the determination of APC resistance caused by the factor V Leiden mutation. | |