Geisinger Medical Laboratories/Geisinger Proven Diagnostics Test Catalog

Order Display Name: Geisinger Epic Procedure Code Or ID CPT Code:  

HEPATITIS C GENOTYPE, PCR

ORDERING INFORMATION:
Geisinger Epic Procedure Code: LAB2444         Geisinger Epic ID: 47178

SPECIMEN COLLECTION
Specimen type:
Plasma
Preferred collection container:
Specimen required:
3-4 mL plasma in one (1) aliquot tube; minimum 2.4 mL of plasma in aliquot. NO SHARED SPECIMENS.
Special notes:
Separate plasma from whole blood within 24 hours of collection. Each molecular test ordered requires its own aliquot tube (e.g. HCGENO requires one 3-4 mL aliquot tube and HEPCR requires one 3-4 mL aliquot tube). Isolated/extracted nucleic acids are acceptable only from CLIA-certified laboratories or a laboratory meeting equivalent requirements as determined by CAP and/or CMS.

SPECIMEN PROCESSING
Specimen processing instructions:
Separate plasma from EDTA whole blood within 24 hours of collection. Centrifuge for 10 minutes at 3000rpm, room temperature. Using a sterile pipet, transfer 3-4 mL of plasma to one (1) labeled, sterile, aliquot tube. 
Transport temperature:
Plasma frozen, -20 to -80°C (preferred). Plasma may also be transported at 2-8°C. Transport EDTA whole blood specimens at 2-8°C.
Specimen stability:
Plasma specimens may be stored at 2-8°C for up to 72 hours; or frozen at -20°C to -80°C up to 6 weeks (preferred). EDTA whole blood specimens can be stored at 2-8°C up to 24 hours prior to centrifugation.
Rejection criteria:
Whole blood specimens must be collected in EDTA tubes. Specimens must be separated with 24 hours of collection, or will be rejected. Improper storage or transport temperature. Stability limits exceeded. Shared or comingled specimens.

TEST DETAILS
Reference interval:
Negative.
Additional information:
The LOD for HCV Genotypes 1-5 is less than or equal to 500 IU/mL. RNA quantitations lower that 500 IU/mL can produce a valid genotype, unable to genotype, or HCV not detected. The LOD for HCV Genotype 6 is less than or equal to 1000 IU/mL. RNA quantitations lower than 1000 IU/mL can produce a valid genotype, unable to genotype, or HCV not detected.
CPT code(s):
87902
Note: The billing party has sole responsibility for CPT coding.  Any questions regarding coding should be directed to the payer being billed.  The CPT codes provided by GML are based on AMA guidelines and are for informational purposes only.
Test includes:
Hepatitis C Genotyping.
Methodology:
Multiplex Reverse-Transcription Polymerase Chain Reaction (RT-PCR)
GenMark Dx eSensor Technology
Synonyms:
HCV genotyping, HCV RNA genotyping
HCV, Hepatitis C virus genotyping
Hepatitis C genotyping
HCGENO
Clinical significance:
This test detects the six major HCV genotypes and their most common subtypes. Hepatitis C genotype is a predictor of therapeutic response to interferon, combination therapy with interferon and ribavirin and newer therapy regimens. All types respond to therapy, but dosage and duration of treatment depend on the individual's genotype. Individuals with non-type-1 are better responders to therapy; however, type 1 individuals do respond but require extended treatment.
Doctoral Director(s:)
Yi Ding MD, PhD    
Review Date: 08/17/2020

Performing Locations

Geisinger Medical Center

Performing laboratory: Molecular Diagnostics
Technical Lead: Lisa Scicchitano
Frequency: Twice weekly, 1st shift
Performed stat? No
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