Geisinger Medical Laboratories/Geisinger Proven Diagnostics Test Catalog

Test Name: Sunquest Test Code Epic Procedure Code Or ID CPT Code:  

T4 T8 LYMPHOCYTE SUBSET PANEL

ORDERING INFORMATION:
Sunquest Test Code:
T4T8      Geisinger Epic Procedure Code: T4T8         Geisinger Epic ID: 15201

SPECIMEN COLLECTION
Specimen type:
Whole blood
Preferred collection container:
Specimen required:
One (1) 4 mL green-top sodium heparin tube whole blood; minimum 3 mL AND one (1) 2 mL lavender-top EDTA tube whole blood; minimum 1.6 mL.
Special notes:
If unable to collect both tube types, testing can be performed on either the sodium heparin tube or the EDTA tube. See stability limits for collection tubes below.

SPECIMEN PROCESSING
Specimen processing instructions:
Do not centrifuge, refrigerate or freeze. 
Transport temperature:
Room temperature. Do not expose to extremes of temperature.
Specimen stability:
Sodium heparin tube stable for 48 hours at room temperature. Lavender (EDTA) tube stable for 48 hours at room temperature.
Rejection criteria:
Specimen stability exceeded. Clotted specimen. Hemolyzed specimen.

TEST DETAILS
Reference interval:
AGE: CD3 % CD3+
CD4%
CD3+
CD8%
CD19 % CD56+
CD16 %
CD4:CD8 RATIO
17- adult 61-88 35-62 13-35 4.0-21 3.0-21 0.8-3.5
10 52-78 25-48 9.0-35.0 8.0-24.0 6.0-27. 0.9-3.4
5 55-78 27-53 19.0-34.0 10.0-31.0 4.0-26.0 0.9-2.6
2 43-76 23-48 14.0-33.0 14.0-44.0 4.0-23.0 0.9-2.9
15 M 39-73 25-50 11.0-32.0 17.0-41.0 3.0-16.0 0.9-3.7
9 M 54-76 31-54 12.0-28.0 15.0-39.0 3.0-17.0 1.3-3.9
5 M 50-77 33-58 13.0-26.0 13.0-35.0 2.0-13.0 1.6-3.8
2 M 48-75 33-58 11.0-25.0 14.0-39.0 2.0-14.0 1.7-3.9
7 D 60-85 41-68 9.0-23.0 4.0-26.0 3.0-23.0 1.3-6.3
0 28-76 17-52 10.0-41.0 5.0-22.0 6.0-58.0 1.0-2.6

AGE: CD3 Absolute CD3+/CD4+ Absolute CD3+/CD8+ Absolute CD19/CD3-
Absolute
CD56+CD16 Absolute
17 - adult 510-2265 330-1520 150-790 33-550 50-400
10 800-3500 400-2100 200-1200 200-600 70-1200
5 700-4200 300-2000 300-1800 200-1600 90-900
2 900-4500 500-2400 300-1600 200-2100 100-1000
15 M 1400-8000 900-5500 400-2300 600-3100 100-1400
9 M 1600-6700 1000-4600 400-2100 600-2700 200-1200
5 M 2400-6900 1400-5100 600-2200 700-2500 100-1000
2 M 2300-6500 1500-5000 500-1600 600-3000 100-1300
7 D 2300-7000 1700-5300 400-1700 60-1900 200-1400
0 600-5000 400-3500 200-1900 40-1100 100-1900

*Adult ranges established by normal study performed at GMC 2002
*Pediatric ranges from : The Journal of Pediatrics Vol. 130(3), March 1997, pp 388-393
Additional information:
Analysis performed according to CDC Guidelines.
CPT code(s):
86355, 86357, 86359, 86360
Note: The billing party has sole responsibility for CPT coding.  Any questions regarding coding should be directed to the payer being billed.  The CPT codes provided by GML are based on AMA guidelines and are for informational purposes only.
Test includes:
Total T Cells, T-helper cells, T- suppressor cells, B cells and NK (natural killer) cells.
Methodology:
Flow Cytometry
Synonyms:
CDC Lymphocyte panel, Immunodeficiency profile, T and B cell subsets
CD3, CD4, CD8, CD19, CD56,
CD4CD8 Lymphocyte Panel
Clinical significance:
Lymphocyte subset anlysis is useful for evaluation of immunocompetency in suspected cases of congenital or acquired immunodeficiency.
Doctoral Director(s:)
Kai Zhang M.D.    
Review Date: 11/14/2018

Performing Locations

Geisinger Medical Center

Performing laboratory: Flow Cytometry
Technical Lead: Michele Adler
Frequency: 1st shift 7am to 5pm. Call 570-271-5261 during hours of operation
Performed stat? No
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*GMC - Geisinger Medical Center; GWV - Geisinger Wyoming Valley Medical Center; GSACH - Geisinger Shamokin Area Community Hospital; GBH - Geisinger Bloomsburg Hospital;
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