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Geisinger Medical Laboratories/Geisinger Proven Diagnostics Test Catalog
LEUKEMIA LYMPHOMA PANEL,FLOW CYTOMETRY |
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ORDERING INFORMATION: |
Geisinger Epic Procedure Code: LAB3388 Geisinger Epic ID: 95697 | |
SPECIMEN COLLECTION |
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Specimen type: |
Whole blood or bone marrow aspirate or lymphoid tissue or fine needle aspirate (FNA) or effusion | |
Specimen required: |
Blood: sodium heparin tube properly filled Bone Marrow Aspirate: 2-3 ml bone marrow in sodium heparin tube, Effusion: Add 10 mL to 1 to 3 sodium heparin tubes Fine Needle Aspirate: Aspirate in RPMI medium Fresh tissue: piece of tissue in RPMI medium |
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Special notes: |
The Geisinger flow cytometry group recommends the following utilization guidelines for peripheral blood flow cytometry testing. a.Lymphocytosis with absolute lymphocyte count (ALC) >5,000/ul in patients >50 years old for CLL and related non-Hodgkin’s lymphoproliferative disorders in leukemic phase. b.History of lymphoproliferative disorders (LPDs) of B- or T-cell lineage regardless of patient age. c.To evaluate abnormal lymphocytes, to rule in or rule out hairy cell leukemia, large granular lymphocytosis (LGLs) or plasma cell leukemia. d.Immunophenotypic characterization of blasts to rule in or rule out acute lymphoblastic leukemia or acute myeloid leukemia. In this setting a hematology consult may have already been initiated. Please be advised that when a patient’s CBC is abnormal and there is uncertainty as to whether flow cytometry testing on blood can help, please do not hesitate to contact one of the pathologists on the flow cytometry at Tel: 570-271-6332. In any of the described settings you may order a peripheral smear review and leave the decision of necessity of flow cytometry up to the pathologist. Also, the pathologist may contact you directly if there are questions about the procedure or indications in a specific case. Do not place FNA or fresh tissue in any other medium than RPMI. | |
| SPECIMEN PROCESSING |
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Specimen processing instructions: |
Do NOT centrifuge. | |
Transport temperature: |
Whole blood and bone marrow aspirate: Soduim heparin tube at room temperature. Effusion, FNA, or tissue:store and ship with a cool pack. Do not place tube directly on ice pack. Do not freeze. |
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Specimen stability: |
Whole blood and bone marrow aspirate: 48 hours at room temperature. Effusion, FNA, or tissue: 72 hours refrigerated in RPMI medium. |
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Rejection criteria: |
Sample stability exceeded, clotted specimens, poor viability, insufficient cells. Specimens in any type of cytology fixative, e.g., Michel’s Solution or Sacoomano fluid. | |
TEST DETAILS |
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Interpretation guide: |
Interpretation of the immunophenotypic findings and correlation with the morphologic feature will be provided in a written pathology report | |
Critical values/courtesy alerts: |
Positive results called on request | |
CPT code(s): |
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| Note: The billing party has sole responsibility for CPT coding. Any questions regarding coding should be directed to the payer being billed. The CPT codes provided by GML are based on AMA guidelines and are for informational purposes only. | ||
Test includes: |
Lymphoma and leukemia markers, slide review, and pathologist interpretation. | |
Methodology: |
Flow Cytometry |
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Clinical significance: |
Immunophenotyping of abnormal cells found in blood, bone marrow and lymphoid tissues is used to aid in the diagnosis of lymphomas, leukemias, multiple myeloma, and myeloproliferative disorders. | |
Doctoral Director(s:) |
Kai Zhang M.D. | |
| Review Date: | 12/14/2022 | |