Geisinger Medical Laboratories/Geisinger Proven Diagnostics Test Catalog

Test Name: Sunquest Test Code Epic Procedure Code Or ID CPT Code:  

ENTEROVIRUS RNA, QUAL.

ORDERING INFORMATION:
Sunquest Test Code:
EVRNA      Geisinger Epic Procedure Code: 87498         Geisinger Epic ID: 34231

SPECIMEN COLLECTION
Specimen type:
CSF (preferred). Plasma, serum, stool, and lesion, rectal, or throat swab also acceptable.
Preferred collection container:
Alternate Collection Container:
Viral/Chlamydia transport (M4 Transport)
Sterile specimen container
6 mL red-top (plain, non-serum separator) tube
3 mL lavender-top (K2 EDTA) tube
Specimen required:
0.7 mL(min 0.3 mL) CSF in sterile container (preferred). 0.7 mL (mins 0.3 mL) serum from red-top tube or plasma from EDTA tube, 1g (min 0.5 g) stool in sterile container and lesion/rectal/throat swab in VCM or M4 Transport also acceptable.
Special notes:
See Specimen Processing Instructions.

SPECIMEN PROCESSING
Specimen processing instructions:
Separate serum or plasma from cells within 2 hours of collection. Stool samples must be sent frozen. 
Transport temperature:
CSF, serum, plasma or swab: Refrigerated. Stool: Frozen.
Specimen stability:
CSF, Serum, Plasma: Room temperature: 48 hours. Refrigerated: 7 days. Frozen: 30 days.
Stool: Room temperature: Unacceptable. Refrigerated: 7 days. Frozen: 30 days.
Swab: Room temperature: 48 hours. Refrigerated: 7 days. Frozen: Unacceptable.
Rejection criteria:
Received past stability, swab received frozen, stool received thawed.

TEST DETAILS
CPT code(s):
87498
Note: The billing party has sole responsibility for CPT coding.  Any questions regarding coding should be directed to the payer being billed.  The CPT codes provided by GML are based on AMA guidelines and are for informational purposes only.
Test includes:
Enterovirus RNA, Qualitative Real-Time PCR
Methodology:
Real-time Polymerase Chain Reaction (PCR)
Synonyms:
Enterovirus
Quest test code 15082
Clinical significance:
Reverse Transcriptase-PCR (RT-PCR) is a highly sensitive method used to detect Enterovirus (EV) RNA from various clinical specimens. This assay targets a conserved region of the EV genome which allows detection of nearly the entire spectrum of human enteroviruses, including the coxsackieviruses, polioviruses and the echoviruses. It does not differentiate among the EV serotypes. The diagnosis of enterovirus infection should not rely solely upon the result of a RT-PCR assay. A positive result should be considered in conjunction with clinical presentation and additional established clinical tests. A negative RT-PCR result indicates the absence of enterovirus RNA in the sample tested and does not exclude the diagnosis of disease.

Performing Locations

Quest Diagnostics

Technical Lead: Michael Weaver    
Frequency: Monday-Sunday morning    
Performed stat? No
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