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Geisinger Medical Laboratories/Geisinger Proven Diagnostics Test Catalog
ENTEROVIRUS RNA, QL REAL TIME PCR |
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ORDERING INFORMATION: |
Geisinger Epic Procedure Code: LAB2220 Geisinger Epic ID: 34231 | |
SPECIMEN COLLECTION |
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Specimen type: |
CSF (preferred). Plasma, serum, stool, and lesion, rectal, or throat swab also acceptable. | |
Preferred collection container: |
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Alternate Collection Container: |
Viral/Chlamydia transport (M4 Transport)
Sterile specimen container
6 mL red-top (plain, non-serum separator) tube
3 mL lavender-top (K2 EDTA) tube
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Specimen required: |
0.7 mL(min 0.3 mL) CSF in sterile container (preferred). 0.7 mL (mins 0.3 mL) serum from red-top tube or plasma from EDTA tube, 1g (min 0.5 g) stool in sterile container and lesion/rectal/throat swab in VCM or M4 Transport also acceptable. | |
Special notes: |
See Specimen Processing Instructions. | |
| SPECIMEN PROCESSING |
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Specimen processing instructions: |
Separate serum or plasma from cells within 2 hours of collection. Stool samples must be sent frozen. | |
Transport temperature: |
CSF, serum, plasma or swab: Refrigerated. Stool: Frozen. | |
Specimen stability: |
CSF, Serum, Plasma: Room temperature: 48 hours. Refrigerated: 7 days. Frozen: 30 days. Stool: Room temperature: Unacceptable. Refrigerated: 7 days. Frozen: 30 days. Swab: Room temperature: 48 hours. Refrigerated: 7 days. Frozen: Unacceptable. |
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Rejection criteria: |
Received past stability, swab received frozen, stool received thawed. | |
TEST DETAILS |
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CPT code(s): |
87498 | |
| Note: The billing party has sole responsibility for CPT coding. Any questions regarding coding should be directed to the payer being billed. The CPT codes provided by GML are based on AMA guidelines and are for informational purposes only. | ||
Test includes: |
Enterovirus RNA, Qualitative Real-Time PCR | |
Methodology: |
Real-time Polymerase Chain Reaction (PCR) |
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Synonyms: |
Enterovirus Quest test code 15082 EVRNA
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Clinical significance: |
Reverse Transcriptase-PCR (RT-PCR) is a highly sensitive method used to detect Enterovirus (EV) RNA from various clinical specimens. This assay targets a conserved region of the EV genome which allows detection of nearly the entire spectrum of human enteroviruses, including the coxsackieviruses, polioviruses and the echoviruses. It does not differentiate among the EV serotypes. The diagnosis of enterovirus infection should not rely solely upon the result of a RT-PCR assay. A positive result should be considered in conjunction with clinical presentation and additional established clinical tests. A negative RT-PCR result indicates the absence of enterovirus RNA in the sample tested and does not exclude the diagnosis of disease. | |